Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
نویسندگان
چکیده
منابع مشابه
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used...
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Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC. Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored dur...
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background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...
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Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these ce...
متن کاملexpression of spermatogonial and pluripotency markers in spermatogonial stem cells after treatment with different culture factors
background: as condition and component of culture determine fate map of spermatogonial stem cells (sscs), the aim of this study was to evaluate of growth factors gdnf, lif and ra on proliferation and differentiation of ssc. materials and methods: sscs were cultured in two groups: the first group gdnf and lif and the second group ra. the number of clumps and colony formation was monitored during...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2007
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2007.1015